Functional single-cell hybridoma screening using droplet-based microfluidics
نویسندگان
چکیده
منابع مشابه
Functional single-cell hybridoma screening using droplet-based microfluidics.
Monoclonal antibodies can specifically bind or even inhibit drug targets and have hence become the fastest growing class of human therapeutics. Although they can be screened for binding affinities at very high throughput using systems such as phage display, screening for functional properties (e.g., the inhibition of a drug target) is much more challenging. Typically these screens require the g...
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Microfluidics allows the manipulation of small quantities of reagents in a high-throughput manner and is therefore highly amenable to single cell characterization and more generally to digital analysis, with applications in fields as varied as genomics, diagnostics, directed evolution, and drug screening. The growing place of microfluidics in biology laboratories encouraged us to develop a ...
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Bioaffinity mass spectrometry screening is a novel approach using non-denaturing electrospray ionization (ESI) mass spectrometry (MS) in identifying drug leads. This screening technique can detect and preserve noncovalent protein-active drug ligand complexes under different physiological conditions. Although there are many successful screening campaigns employing this technique, the big challen...
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A critical early step in drug discovery is the screening of a chemical library. Typically, promising compounds are identified in a primary screen and then more fully characterized in a dose-response analysis with 7-10 data points per compound. Here, we describe a robust microfluidic approach that increases the number of data points to approximately 10,000 per compound. The system exploits Taylo...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2012
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1204514109